SARS-CoV-2 viral shedding characteristics and potential evidence for the priority for faecal specimen testing in diagnosis yuan2021sars

This study investigates the shedding of SARS-CoV-2 RNA across multiple specimen types-including stool, respiratory secretions, urine, and serum-in both symptomatic and asymptomatic COVID-19 patients. It evaluates viral load dynamics and time to clearance across specimen types.

Analytes

nasopharyngeal_swab_SARSCoV2_ORF1ab_symptomatic

This entry describes RT-qPCR detection of SARS-CoV-2 RNA (Ct < 40 considered positive) targeting the ORF1ab gene in nasopharyngeal_swab from symptomatic individuals.

Biomarker: SARS-CoV-2
Specimen: nasopharyngeal swab
Units: cycle threshold
Gene target: ORF1ab
Participants: 6
Negative samples: 56
Positive samples (not quantifiable): 0
Quantifiable samples: 28
Limit of quantification: unknown
Limit of detection: 40

nasopharyngeal_swab_SARSCoV2_ORF1ab_asymptomatic

This entry describes RT-qPCR detection of SARS-CoV-2 RNA (Ct < 40 considered positive) targeting the ORF1ab gene in nasopharyngeal_swab from asymptomatic individuals.

Biomarker: SARS-CoV-2
Specimen: nasopharyngeal swab
Units: cycle threshold
Gene target: ORF1ab
Participants: 4
Negative samples: 31
Positive samples (not quantifiable): 0
Quantifiable samples: 2
Limit of quantification: unknown
Limit of detection: 40

nasopharyngeal_swab_SARSCoV2_N_symptomatic

This entry describes RT-qPCR detection of SARS-CoV-2 RNA (Ct < 40 considered positive) targeting the N gene in nasopharyngeal_swab from symptomatic individuals.

Biomarker: SARS-CoV-2
Specimen: nasopharyngeal swab
Units: cycle threshold
Gene target: N
Participants: 6
Negative samples: 52
Positive samples (not quantifiable): 0
Quantifiable samples: 32
Limit of quantification: unknown
Limit of detection: 40

nasopharyngeal_swab_SARSCoV2_N_asymptomatic

This entry describes RT-qPCR detection of SARS-CoV-2 RNA (Ct < 40 considered positive) targeting the N gene in nasopharyngeal_swab from asymptomatic individuals.

Biomarker: SARS-CoV-2
Specimen: nasopharyngeal swab
Units: cycle threshold
Gene target: N
Participants: 4
Negative samples: 30
Positive samples (not quantifiable): 0
Quantifiable samples: 3
Limit of quantification: unknown
Limit of detection: 40

stool_SARSCoV2_ORF1ab_symptomatic

This entry describes RT-qPCR detection of SARS-CoV-2 RNA (Ct < 40 considered positive) targeting the ORF1ab gene in stool from symptomatic individuals.

Biomarker: SARS-CoV-2
Specimen: stool
Units: cycle threshold
Gene target: ORF1ab
Participants: 6
Negative samples: 24
Positive samples (not quantifiable): 0
Quantifiable samples: 22
Limit of quantification: unknown
Limit of detection: 40

stool_SARSCoV2_ORF1ab_asymptomatic

This entry describes RT-qPCR detection of SARS-CoV-2 RNA (Ct < 40 considered positive) targeting the ORF1ab gene in stool from asymptomatic individuals.

Biomarker: SARS-CoV-2
Specimen: stool
Units: cycle threshold
Gene target: ORF1ab
Participants: 4
Negative samples: 8
Positive samples (not quantifiable): 0
Quantifiable samples: 28
Limit of quantification: unknown
Limit of detection: 40

stool_SARSCoV2_N_symptomatic

This entry describes RT-qPCR detection of SARS-CoV-2 RNA (Ct < 40 considered positive) targeting the N gene in stool from symptomatic individuals.

Biomarker: SARS-CoV-2
Specimen: stool
Units: cycle threshold
Gene target: N
Participants: 6
Negative samples: 22
Positive samples (not quantifiable): 0
Quantifiable samples: 24
Limit of quantification: unknown
Limit of detection: 40

stool_SARSCoV2_N_asymptomatic

This entry describes RT-qPCR detection of SARS-CoV-2 RNA (Ct < 40 considered positive) targeting the N gene in stool from asymptomatic individuals.

Biomarker: SARS-CoV-2
Specimen: stool
Units: cycle threshold
Gene target: N
Participants: 4
Negative samples: 8
Positive samples (not quantifiable): 0
Quantifiable samples: 28
Limit of quantification: unknown
Limit of detection: 40

serum_SARSCoV2_ORF1ab_symptomatic

This entry describes RT-qPCR detection of SARS-CoV-2 RNA (Ct < 40 considered positive) targeting the ORF1ab gene in serum from symptomatic individuals.

Biomarker: SARS-CoV-2
Specimen: serum
Units: cycle threshold
Gene target: ORF1ab
Participants: 3
Negative samples: 3
Positive samples (not quantifiable): 0
Quantifiable samples: 0
Limit of quantification: unknown
Limit of detection: 40

serum_SARSCoV2_ORF1ab_asymptomatic

This entry describes RT-qPCR detection of SARS-CoV-2 RNA (Ct < 40 considered positive) targeting the ORF1ab gene in serum from asymptomatic individuals.

Biomarker: SARS-CoV-2
Specimen: serum
Units: cycle threshold
Gene target: ORF1ab
Participants: 1
Negative samples: 1
Positive samples (not quantifiable): 0
Quantifiable samples: 0
Limit of quantification: unknown
Limit of detection: 40

serum_SARSCoV2_N_symptomatic

This entry describes RT-qPCR detection of SARS-CoV-2 RNA (Ct < 40 considered positive) targeting the N gene in serum from symptomatic individuals.

Biomarker: SARS-CoV-2
Specimen: serum
Units: cycle threshold
Gene target: N
Participants: 3
Negative samples: 3
Positive samples (not quantifiable): 0
Quantifiable samples: 0
Limit of quantification: unknown
Limit of detection: 40

serum_SARSCoV2_N_asymptomatic

This entry describes RT-qPCR detection of SARS-CoV-2 RNA (Ct < 40 considered positive) targeting the N gene in serum from asymptomatic individuals.

Biomarker: SARS-CoV-2
Specimen: serum
Units: cycle threshold
Gene target: N
Participants: 1
Negative samples: 1
Positive samples (not quantifiable): 0
Quantifiable samples: 0
Limit of quantification: unknown
Limit of detection: 40

urine_SARSCoV2_ORF1ab_symptomatic

This entry describes RT-qPCR detection of SARS-CoV-2 RNA (Ct < 40 considered positive) targeting the ORF1ab gene in urine from symptomatic individuals.

Biomarker: SARS-CoV-2
Specimen: urine
Units: cycle threshold
Gene target: ORF1ab
Participants: 3
Negative samples: 6
Positive samples (not quantifiable): 0
Quantifiable samples: 0
Limit of quantification: unknown
Limit of detection: 40

urine_SARSCoV2_ORF1ab_asymptomatic

This entry describes RT-qPCR detection of SARS-CoV-2 RNA (Ct < 40 considered positive) targeting the ORF1ab gene in urine from asymptomatic individuals.

Biomarker: SARS-CoV-2
Specimen: urine
Units: cycle threshold
Gene target: ORF1ab
Participants: 2
Negative samples: 2
Positive samples (not quantifiable): 0
Quantifiable samples: 0
Limit of quantification: unknown
Limit of detection: 40

urine_SARSCoV2_N_symptomatic

This entry describes RT-qPCR detection of SARS-CoV-2 RNA (Ct < 40 considered positive) targeting the N gene in urine from symptomatic individuals.

Biomarker: SARS-CoV-2
Specimen: urine
Units: cycle threshold
Gene target: N
Participants: 3
Negative samples: 6
Positive samples (not quantifiable): 0
Quantifiable samples: 0
Limit of quantification: unknown
Limit of detection: 40

urine_SARSCoV2_N_asymptomatic

This entry describes RT-qPCR detection of SARS-CoV-2 RNA (Ct < 40 considered positive) targeting the N gene in urine from asymptomatic individuals.

Biomarker: SARS-CoV-2
Specimen: urine
Units: cycle threshold
Gene target: N
Participants: 2
Negative samples: 2
Positive samples (not quantifiable): 0
Quantifiable samples: 0
Limit of quantification: unknown
Limit of detection: 40