Clinical features and viral load variations of Mpox: a retrospective study in Chongqing, China yang2024clinical

Retrospective case series of nine PCR-confirmed mpox (MPXV) infections among MSM men admitted to Chongqing Public Health Medical Center (Chongqing, China) from September 2022 to October 2023. MPXV DNA was detected by qPCR (DAAN Gene kit on Bio-Rad CFX96 after EXM6000 magnetic-bead extraction) and results were reported as Ct values across multiple specimen types (skin lesions, oropharyngeal swab, nasopharyngeal swab, anus swab, and feces/stool). CSV figure-derived Ct time series were provided and extracted (70 rows total), with time in days relative to symptom onset.

Analytes

nasopharyngeal_swab

MPXV DNA detection by quantitative real-time PCR (qPCR) reported as Ct values from nasopharyngeal swab specimens; DNA extracted using EXM6000 magnetic bead method; PCR on Bio-Rad CFX96 using DAAN Gene MPXV nucleic acid testing kit.

observations for nasopharyngeal_swab

Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.

Biomarker: mpox
Specimen: nasopharyngeal swab
Units: cycle threshold
Participants: 5
Negative samples: 12
Positive samples (not quantifiable): 0
Quantifiable samples: 5
Limit of quantification: unknown
Limit of detection: 40

skin

MPXV DNA detection by quantitative real-time PCR (qPCR) reported as Ct values from skin lesion specimens; DNA extracted using EXM6000 magnetic bead method; PCR on Bio-Rad CFX96 using DAAN Gene MPXV nucleic acid testing kit.

observations for skin

Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.

Biomarker: mpox
Specimen: skin lesion
Units: cycle threshold
Participants: 9
Negative samples: 6
Positive samples (not quantifiable): 0
Quantifiable samples: 20
Limit of quantification: unknown
Limit of detection: 40

stool

MPXV DNA detection by quantitative real-time PCR (qPCR) reported as Ct values from feces/stool specimens; DNA extracted using EXM6000 magnetic bead method; PCR on Bio-Rad CFX96 using DAAN Gene MPXV nucleic acid testing kit.

observations for stool

Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.

Biomarker: mpox
Specimen: stool
Units: cycle threshold
Participants: 1
Negative samples: 7
Positive samples (not quantifiable): 0
Quantifiable samples: 2
Limit of quantification: unknown
Limit of detection: 40

anus

MPXV DNA detection by quantitative real-time PCR (qPCR) reported as Ct values from anus (anorectal) swab specimens; DNA extracted using EXM6000 magnetic bead method; PCR on Bio-Rad CFX96 using DAAN Gene MPXV nucleic acid testing kit.

observations for anus

Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.

Biomarker: mpox
Specimen: anorectal swab
Units: cycle threshold
Participants: 4
Negative samples: 5
Positive samples (not quantifiable): 0
Quantifiable samples: 4
Limit of quantification: unknown
Limit of detection: 40

oropharyngeal_swab

MPXV DNA detection by quantitative real-time PCR (qPCR) reported as Ct values from oropharyngeal (throat) swab specimens; DNA extracted using EXM6000 magnetic bead method; PCR on Bio-Rad CFX96 using DAAN Gene MPXV nucleic acid testing kit.

observations for oropharyngeal_swab

Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.

Biomarker: mpox
Specimen: oropharyngeal swab
Units: cycle threshold
Participants: 2
Negative samples: 3
Positive samples (not quantifiable): 0
Quantifiable samples: 6
Limit of quantification: unknown
Limit of detection: 40