Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia woo2005characterization
Study reporting discovery and characterization of human coronavirus HKU1 (CoV-HKU1) associated with pneumonia in Hong Kong. An index case (71-year-old Chinese man) had serial nasopharyngeal aspirates tested by quantitative RT-PCR targeting the coronavirus RNA-dependent RNA polymerase (pol) gene fragment, showing high viral loads early in illness followed by undetectable results later. Screening of 400 SARS-CoV-negative nasopharyngeal aspirates from respiratory illness cases during the 2003 SARS period found one additional CoV-HKU1-positive pneumonia case (35-year-old woman).
Analytes
nasopharyngeal_aspirate_coronavirus_HKU1_pol_gc_ml
Real-time quantitative RT-PCR (SYBR Green I; Roche LightCycler) for CoV-HKU1 using specific primers amplifying a 440-bp fragment of the RNA-dependent RNA polymerase (pol) gene; quantification via plasmid standard curve and melting curve analysis for specificity.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.