Shedding of norovirus in symptomatic and asymptomatic infections teunis2015shedding

Longitudinal study of norovirus GII.4 faecal shedding during four nosocomial outbreaks (2009-2011) in a tertiary-care hospital and three nursing homes in The Netherlands. Real-time quantitative RT-PCR was used to measure norovirus genome concentrations in stool from symptomatic and asymptomatic patients and healthcare workers. The study includes 102 subjects with 230 faecal samples overall; data provided here include the CSV subset of 74 sampled measurements (with both Ct and concentration values). Ct readings were done once and calibrated against an RNA standard; Ct=40 was considered the diagnostic detection limit. The analysis compared shedding dynamics (peak, time-to-peak, duration, AUC) between symptomatic and asymptomatic infections.

Analytes

stool_norovirus_ct_symptomatic

Cycle threshold (Ct) values from quantitative real-time RT-PCR for norovirus (GII.4) measured in stool from symptomatic subjects. Ct readings were done once; calibration used a run-off transcript standard. Ct=40 was considered the diagnostic detection limit.

Biomarker: norovirus
Specimen: stool
Units: cycle threshold
Participants: 70
Negative samples: 46
Positive samples (not quantifiable): 0
Quantifiable samples: 115
Limit of quantification: unknown
Limit of detection: 40

stool_norovirus_ct_asymptomatic

Cycle threshold (Ct) values from quantitative real-time RT-PCR for norovirus (GII.4) measured in stool from asymptomatic subjects. Ct readings were done once; calibration used a run-off transcript standard. Ct=40 was considered the diagnostic detection limit.

Biomarker: norovirus
Specimen: stool
Units: cycle threshold
Participants: 32
Negative samples: 18
Positive samples (not quantifiable): 0
Quantifiable samples: 46
Limit of quantification: unknown
Limit of detection: 40

stool_norovirus_concentration_symptomatic

Quantified norovirus genome concentrations (genome copies per wet gram) derived from real-time RT-PCR for stool samples from symptomatic subjects (calibrated against run-off transcript standard). Concentrations are reported as genome copies per wet gram of faeces.

Biomarker: norovirus
Specimen: stool
Units: gc/wet gram
Participants: 70
Negative samples: 46
Positive samples (not quantifiable): 0
Quantifiable samples: 115
Limit of quantification: unknown
Limit of detection: unknown

stool_norovirus_concentration_asymptomatic

Quantified norovirus genome concentrations (genome copies per wet gram) derived from real-time RT-PCR for stool samples from asymptomatic subjects (calibrated against run-off transcript standard). Concentrations are reported as genome copies per wet gram of faeces.

Biomarker: norovirus
Specimen: stool
Units: gc/wet gram
Participants: 32
Negative samples: 18
Positive samples (not quantifiable): 0
Quantifiable samples: 46
Limit of quantification: unknown
Limit of detection: unknown