Shedding of norovirus in symptomatic and asymptomatic infections teunis2015shedding
Longitudinal study of norovirus GII.4 faecal shedding during four nosocomial outbreaks (2009-2011) in a tertiary-care hospital and three nursing homes in The Netherlands. Real-time quantitative RT-PCR was used to measure norovirus genome concentrations in stool from symptomatic and asymptomatic patients and healthcare workers. The study includes 102 subjects with 230 faecal samples overall; data provided here include the CSV subset of 74 sampled measurements (with both Ct and concentration values). Ct readings were done once and calibrated against an RNA standard; Ct=40 was considered the diagnostic detection limit. The analysis compared shedding dynamics (peak, time-to-peak, duration, AUC) between symptomatic and asymptomatic infections.
Analytes
stool_norovirus_ct_symptomatic
Cycle threshold (Ct) values from quantitative real-time RT-PCR for norovirus (GII.4) measured in stool from symptomatic subjects. Ct readings were done once; calibration used a run-off transcript standard. Ct=40 was considered the diagnostic detection limit.
Fitted by censored maximum likelihood. The red line is the median individual; the shaded region is the full range of a simulated cohort drawn from the fitted population, so it shows what simulating from this dataset would produce rather than a confidence interval, with dashed lines at the central 95%. Open triangles are non-detects, drawn at the censoring limit and entering the fit as "below this value" rather than being dropped. See the modelling methods for what these estimates do and do not support.
stool_norovirus_ct_asymptomatic
Cycle threshold (Ct) values from quantitative real-time RT-PCR for norovirus (GII.4) measured in stool from asymptomatic subjects. Ct readings were done once; calibration used a run-off transcript standard. Ct=40 was considered the diagnostic detection limit.
Fitted by censored maximum likelihood. The red line is the median individual; the shaded region is the full range of a simulated cohort drawn from the fitted population, so it shows what simulating from this dataset would produce rather than a confidence interval, with dashed lines at the central 95%. Open triangles are non-detects, drawn at the censoring limit and entering the fit as "below this value" rather than being dropped. See the modelling methods for what these estimates do and do not support.
stool_norovirus_concentration_symptomatic
Quantified norovirus genome concentrations (genome copies per wet gram) derived from real-time RT-PCR for stool samples from symptomatic subjects (calibrated against run-off transcript standard). Concentrations are reported as genome copies per wet gram of faeces.
Fitted by censored maximum likelihood. The red line is the median individual; the shaded region is the full range of a simulated cohort drawn from the fitted population, so it shows what simulating from this dataset would produce rather than a confidence interval, with dashed lines at the central 95%. Open triangles are non-detects, drawn at the censoring limit and entering the fit as "below this value" rather than being dropped. See the modelling methods for what these estimates do and do not support.
stool_norovirus_concentration_asymptomatic
Quantified norovirus genome concentrations (genome copies per wet gram) derived from real-time RT-PCR for stool samples from asymptomatic subjects (calibrated against run-off transcript standard). Concentrations are reported as genome copies per wet gram of faeces.
Fitted by censored maximum likelihood. The red line is the median individual; the shaded region is the full range of a simulated cohort drawn from the fitted population, so it shows what simulating from this dataset would produce rather than a confidence interval, with dashed lines at the central 95%. Open triangles are non-detects, drawn at the censoring limit and entering the fit as "below this value" rather than being dropped. See the modelling methods for what these estimates do and do not support.