Exploring the Potential of Next-Generation Sequencing in Detection of Respiratory Viruses prachayangprecha2014exploring

Evaluation study of a sequence-independent next-generation sequencing approach versus routine diagnostic real-time RT-PCR for detecting respiratory viruses in nasopharyngeal aspirates from 81 Thai children with respiratory disease. Figure-derived CSV data provide per-patient real-time RT-PCR cycle threshold (Ct) values at a single timepoint (time=0; reference event not specified in the provided excerpt) for rhinovirus, enterovirus, human metapneumovirus, and bocavirus.

Analytes

rhinovirus_ct

Real-time RT-PCR cycle threshold (Ct) measurement for rhinovirus from nasopharyngeal aspirate (figure-derived CSV). Assay gene target not specified in provided data excerpt.

observations for rhinovirus_ct

Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.

Biomarker: rhinovirus
Specimen: nasopharyngeal aspirate
Units: cycle threshold
Participants: 23
Negative samples: 0
Positive samples (not quantifiable): 0
Quantifiable samples: 23
Limit of quantification: unknown
Limit of detection: unknown

enterovirus_ct

Real-time RT-PCR cycle threshold (Ct) measurement for enterovirus from nasopharyngeal aspirate (figure-derived CSV). Assay gene target not specified in provided data excerpt.

observations for enterovirus_ct

Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.

Biomarker: enterovirus
Specimen: nasopharyngeal aspirate
Units: cycle threshold
Participants: 15
Negative samples: 0
Positive samples (not quantifiable): 0
Quantifiable samples: 15
Limit of quantification: unknown
Limit of detection: unknown

metapneumovirus_ct

Real-time RT-PCR cycle threshold (Ct) measurement for human metapneumovirus from nasopharyngeal aspirate (figure-derived CSV). Assay gene target not specified in provided data excerpt.

observations for metapneumovirus_ct

Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.

Biomarker: metapneumovirus
Specimen: nasopharyngeal aspirate
Units: cycle threshold
Participants: 3
Negative samples: 0
Positive samples (not quantifiable): 0
Quantifiable samples: 3
Limit of quantification: unknown
Limit of detection: unknown

bocavirus_ct

Real-time RT-PCR cycle threshold (Ct) measurement for bocavirus from nasopharyngeal aspirate (figure-derived CSV). Assay gene target not specified in provided data excerpt.

observations for bocavirus_ct

Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.

Biomarker: bocavirus
Specimen: nasopharyngeal aspirate
Units: cycle threshold
Participants: 21
Negative samples: 0
Positive samples (not quantifiable): 0
Quantifiable samples: 21
Limit of quantification: unknown
Limit of detection: unknown