Prevalence and duration of SARS-CoV-2 fecal shedding in breastfeeding dyads following maternal COVID-19 diagnosis pace2024prevalence

This study examined whether and to what extent SARS-CoV-2 is detectable in the feces of lactating women and their breastfed infants following maternal COVID-19 diagnosis. A total of 57 maternal-infant dyads provided maternal and/or infant fecal samples, including 33 dyads in the COVID-19 group and 24 dyads in the healthy group. Fecal samples were collected from each mother and child in both the groups and telephone surveys administered during the first week following enrollment (1, 2-6 and 7 day) and again around 2, 3, 4, and 8 wk following enrollment for COVID-19 group, and two separate days during the first week after enrollment (1, 7 day) and again around 3 and 8 wk for healthy group. Since the author didn’t provide the specific time information in the paper, we adopted mean value of time period as our data (4d for 2-6d, 11d for 2wk, 18d for 3wk, 25d for 4wk, 53d for 8wk).

Analytes

stool_SARSCoV2

RNA isolated from fecal samples (including negative and positive controls) was used as the input for Quick SARS-CoV-2 multiplex reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assay (cat. R3013, Zymo Research).

Biomarker: SARS-CoV-2
Specimen: stool
Units: cycle threshold
Participants: 64
Negative samples: 313
Positive samples (not quantifiable): 0
Quantifiable samples: 56
Limit of quantification: unknown
Limit of detection: 40