The role of human Metapneumovirus genetic diversity and nasopharyngeal viral load on symptom severity in adults oong2018role
Adult outpatient acute upper respiratory tract infection cohort recruited at the University of Malaya Medical Centre (Kuala Lumpur, Malaysia) between February 2012 and May 2014. Nasopharyngeal swabs were collected in universal transport media and tested for human metapneumovirus (HMPV). HMPV-positive samples were genotyped/sub-lineaged by sequencing (F and G genes), and viral load was quantified using an improved one-step RT-qPCR assay targeting the HMPV M2 gene. Measurements are reported as RNA copies per microliter of sample, with sampling time in days since symptom onset. CSV-provided data include 75 per-sample viral load measurements with participant-level attributes (genotype/sub-lineage, sex, ethnicity, age).
Analytes
nasopharyngeal_swab_metapneumovirus_viral_load
Quantification of HMPV RNA from nasopharyngeal swabs using a quantitative one-step RT-qPCR assay with newly designed primers/probe targeting the conserved M2 gene region; run on ABI ViiA7. Standard curve generated from 10-fold dilution series (2.0×10^1 to 2.0×10^6 RNA copies/μL). Specimens collected in universal transport media (Copan). Lowest quantifiable concentration estimated at ~13 RNA copies/μL.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.