A Severe Form of Mpox Infection and the Current Epidemiological Status in Romania negru2025severe
Retrospective descriptive study of mpox epidemiology in Romania (national surveillance in 2022–2023; 47 confirmed cases in 2022) plus a detailed severe-case longitudinal shedding description from one immunocompromised male patient with AIDS treated with tecovirimat. Monkeypox virus (MPXV) was detected by qPCR (genesig Advanced kit) from multiple specimen types collected serially over ~5 months; the figure-derived CSV provides Ct values over time for serum, skin lesion, saliva, urine, rectal swab, nasal, and pharyngeal specimens for PatientID 1.
Analytes
serum
MPXV genome detection by qPCR using monkeypox virus genesig Advanced Kit (Primerdesign, UK) on nucleic acid extracted (QIAamp DSP Virus Kit) from clinical samples collected in viral transport medium (VTM) and run on a Bio-Rad CFX96; CSV values are Ct (cycle threshold) over time.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
skin
MPXV genome detection by qPCR using monkeypox virus genesig Advanced Kit (Primerdesign, UK) on nucleic acid extracted (QIAamp DSP Virus Kit) from clinical samples collected in viral transport medium (VTM) and run on a Bio-Rad CFX96; CSV values are Ct (cycle threshold) over time.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
saliva
MPXV genome detection by qPCR using monkeypox virus genesig Advanced Kit (Primerdesign, UK) on nucleic acid extracted (QIAamp DSP Virus Kit) from clinical samples collected in viral transport medium (VTM) and run on a Bio-Rad CFX96; CSV values are Ct (cycle threshold) over time.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
urine
MPXV genome detection by qPCR using monkeypox virus genesig Advanced Kit (Primerdesign, UK) on nucleic acid extracted (QIAamp DSP Virus Kit) from clinical samples collected in viral transport medium (VTM) and run on a Bio-Rad CFX96; CSV values are Ct (cycle threshold) over time.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
rectal_swab
MPXV genome detection by qPCR using monkeypox virus genesig Advanced Kit (Primerdesign, UK) on nucleic acid extracted (QIAamp DSP Virus Kit) from clinical samples collected in viral transport medium (VTM) and run on a Bio-Rad CFX96; CSV values are Ct (cycle threshold) over time.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
nasal
MPXV genome detection by qPCR using monkeypox virus genesig Advanced Kit (Primerdesign, UK) on nucleic acid extracted (QIAamp DSP Virus Kit) from clinical samples collected in viral transport medium (VTM) and run on a Bio-Rad CFX96; CSV values are Ct (cycle threshold) over time.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
pharyngeal
MPXV genome detection by qPCR using monkeypox virus genesig Advanced Kit (Primerdesign, UK) on nucleic acid extracted (QIAamp DSP Virus Kit) from clinical samples collected in viral transport medium (VTM) and run on a Bio-Rad CFX96; CSV values are Ct (cycle threshold) over time.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.