New challenges in human monkeypox outside Africa: A review and case report from Italy mileto2022new
Case report (with review) describing an Italian adult male diagnosed with mpox (monkeypox virus, MPXV) in Milan, Italy (May 2022). Serial sampling from multiple body sites was tested by real-time PCR (Ct values reported) and viral isolation in Vero E6 cells; Table 1/figure CSV provide Ct time series by days from symptom onset for several specimen types (oropharyngeal swab, ulcerated perianal lesion, rectal/anal swab, foot lesion, plasma, seminal fluid, urine).
Analytes
ct_oropharyngeal_swab
MPXV DNA detection by real-time PCR reported as cycle threshold (Ct) on oropharyngeal swab; Orthopoxvirus screening PCR (RealStar Orthopoxvirus PCR Kit 1.0, altona DIAGNOSTICS, targeting variola virus and non-variola Orthopoxvirus species) with MPXV confirmation by a specific homemade real-time PCR on screening-positive samples. Ct=40 used as PCR negative cut-off.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
ct_ulcerated_perianal_lesion
MPXV DNA detection by real-time PCR reported as Ct on ulcerated perianal lesion swab; Orthopoxvirus screening PCR (RealStar Orthopoxvirus PCR Kit 1.0, altona DIAGNOSTICS) with MPXV confirmation by homemade real-time PCR on screening-positive samples. Ct=40 used as PCR negative cut-off.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
ct_anorectal_swab
MPXV DNA detection by real-time PCR reported as Ct on anal swab; Orthopoxvirus screening PCR (RealStar Orthopoxvirus PCR Kit 1.0, altona DIAGNOSTICS) with MPXV confirmation by homemade real-time PCR on screening-positive samples. Ct=40 used as PCR negative cut-off.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
ct_foot_lesion
MPXV DNA detection by real-time PCR reported as Ct on foot lesion sample; Orthopoxvirus screening PCR (RealStar Orthopoxvirus PCR Kit 1.0, altona DIAGNOSTICS) with MPXV confirmation by homemade real-time PCR on screening-positive samples. Ct=40 used as PCR negative cut-off.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
ct_plasma
MPXV DNA detection by real-time PCR reported as Ct in plasma; Orthopoxvirus screening PCR (RealStar Orthopoxvirus PCR Kit 1.0, altona DIAGNOSTICS) with MPXV confirmation by homemade real-time PCR on screening-positive samples. Ct=40 used as PCR negative cut-off.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
ct_seminal_fluid
MPXV DNA detection by real-time PCR reported as Ct in seminal fluid; Orthopoxvirus screening PCR (RealStar Orthopoxvirus PCR Kit 1.0, altona DIAGNOSTICS) with MPXV confirmation by homemade real-time PCR on screening-positive samples. Ct=40 used as PCR negative cut-off.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
ct_urine
MPXV DNA detection by real-time PCR reported as Ct in urine; Orthopoxvirus screening PCR (RealStar Orthopoxvirus PCR Kit 1.0, altona DIAGNOSTICS) with MPXV confirmation by homemade real-time PCR on screening-positive samples. Ct=40 used as PCR negative cut-off.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.