Diagnostic performance of a field-deployable iiPCR platform and viral load dynamics of MPXV Clade IIb in a cohort with high prevalence of HIV liu2026diagnostic
Prospective single-center study (Kaohsiung Medical University Hospital, Taiwan) enrolling 24 qPCR-confirmed mpox (MPXV Clade IIb) patients (2023–2024) to evaluate the POCKIT Central MPXV iiPCR platform versus reference qPCR and to describe MPXV viral load dynamics by specimen type using qPCR Ct values relative to days post-symptom onset. Specimens included lesion-derived materials (pus, vesicular fluid, skin/oral swabs), nasopharyngeal swabs, urine, and plasma. qPCR targeted the MPXV G2R (TNF receptor) gene; iiPCR used manufacturer reagents on an automated sample-to-answer platform. CSV-provided data contain per-patient, per-day qPCR Ct measurements (including negatives) for multiple specimen types timed by days post-symptom onset.
Analytes
pus
MPXV qPCR cycle threshold (Ct) values measured from lesion-derived pus specimens; qPCR performed after nucleic acid extraction (QIAamp MinElute Virus Spin Kit) using a real-time PCR assay targeting MPXV G2R (TNF receptor gene) on an Mx3000P instrument. Time is reported as days post-symptom onset.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
nasopharyngeal_swab
MPXV qPCR cycle threshold (Ct) values measured from nasopharyngeal swab specimens; qPCR performed after nucleic acid extraction (QIAamp MinElute Virus Spin Kit) using a real-time PCR assay targeting MPXV G2R (TNF receptor gene) on an Mx3000P instrument. Time is reported as days post-symptom onset.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
vesicular_fluid
MPXV qPCR cycle threshold (Ct) values measured from lesion-derived vesicular fluid specimens; qPCR performed after nucleic acid extraction (QIAamp MinElute Virus Spin Kit) using a real-time PCR assay targeting MPXV G2R (TNF receptor gene) on an Mx3000P instrument. Time is reported as days post-symptom onset.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
skin_swab_foreskin
MPXV qPCR cycle threshold (Ct) values measured from a foreskin skin swab specimen; qPCR performed after nucleic acid extraction (QIAamp MinElute Virus Spin Kit) using a real-time PCR assay targeting MPXV G2R (TNF receptor gene) on an Mx3000P instrument. Time is reported as days post-symptom onset.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
urine
MPXV qPCR cycle threshold (Ct) values measured from urine specimens; qPCR performed after nucleic acid extraction (QIAamp MinElute Virus Spin Kit) using a real-time PCR assay targeting MPXV G2R (TNF receptor gene) on an Mx3000P instrument. Time is reported as days post-symptom onset.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
plasma
MPXV qPCR cycle threshold (Ct) values measured from plasma specimens; qPCR performed after nucleic acid extraction (QIAamp MinElute Virus Spin Kit) using a real-time PCR assay targeting MPXV G2R (TNF receptor gene) on an Mx3000P instrument. Time is reported as days post-symptom onset.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
skin_swab
MPXV qPCR cycle threshold (Ct) values measured from skin swab specimens; qPCR performed after nucleic acid extraction (QIAamp MinElute Virus Spin Kit) using a real-time PCR assay targeting MPXV G2R (TNF receptor gene) on an Mx3000P instrument. Time is reported as days post-symptom onset.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
mucosal_swab_oral
MPXV qPCR cycle threshold (Ct) values measured from oral mucosal swab specimens; qPCR performed after nucleic acid extraction (QIAamp MinElute Virus Spin Kit) using a real-time PCR assay targeting MPXV G2R (TNF receptor gene) on an Mx3000P instrument. Time is reported as days post-symptom onset.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.