Monkeypox virus isolation from a semen sample collected in the early phase of infection in a patient with prolonged seminal viral shedding lapa2022monkeypox
Case report from Rome, Italy (National Institute for Infectious Diseases ‘Lazzaro Spallanzani’) describing longitudinal mpox (monkeypox virus) DNA detection by real-time PCR in plasma, urine, semen, and rash/skin lesions from a single confirmed patient, with sampling days 5–19 after symptom onset. The report includes seminal shedding with Ct/Cq values and notes isolation of replication-competent virus from semen collected on day 6 after symptom onset using Vero E6 cells.
Analytes
semen
Mpox virus DNA detection in semen by real-time PCR; values are quantification cycle (Cq) reported for positive results (cutoff cycle threshold 45; thresholds ≥42 retested for confirmation).
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
rash_or_skin_lesion
Clinical presence/absence of rash or skin lesions recorded longitudinally (not a PCR Ct measurement).
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
urine
Mpox virus DNA detection in urine by real-time PCR; results reported as negative/not detected (no Cq values provided for urine in CSV).
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
plasma
Mpox virus DNA detection in plasma by real-time PCR; values are quantification cycle (Cq) when positive; otherwise reported as negative.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.