Viral Load Kinetics of SARS-CoV-2 Infection in First Two Patients in Korea kim2020viral
The authors present viral load kinetics for the first two confirmed COVID-19 patients with mild to moderate illness in Korea. Swabs, sputum, serum, plasma, urine, and stool samples were collected throughout the illness. Cycle threshold (Ct) values were quantified using rRT-PCR targeting the RdRp and E genes. Data were obtained from the supplementary material.
Analytes
sputum_SARSCoV2_RdRp_VL
Cycle threshold (Ct) values were quantified using rRT-PCR targeting the RdRp gene in sputum samples. Ct values were then converted into estimated viral copies per mL, with a detection limit of 2,690 copies/mL. The RNA copy number was calculated by the authors using a standard curve derived from Ct values of plasmid DNA, as noted in the supplementary material.
Biomarker: SARS-CoV-2
Specimen:
sputum
Units: gc/mL
Gene target: RdRp
Participants: 2
Negative samples: 10
Positive samples (not quantifiable): 0
Quantifiable samples: 10
Limit of quantification: 2690
Limit of detection: unknown
swab_SARSCoV2_RdRp_VL
Cycle threshold (Ct) values were quantified using rRT-PCR targeting the RdRp gene in swab samples. Ct values were then converted into estimated viral copies per mL, with a detection limit of 2,690 copies/mL. The RNA copy number was calculated by the authors using a standard curve derived from Ct values of plasmid DNA, as noted in the supplementary material.
Biomarker: SARS-CoV-2
Specimen:
nasopharyngeal swab and oropharyngeal swab
Units: gc/mL
Gene target: RdRp
Participants: 2
Negative samples: 11
Positive samples (not quantifiable): 0
Quantifiable samples: 15
Limit of quantification: 2690
Limit of detection: unknown
swab_SARSCoV2_E_Ct
Cycle threshold (Ct) values were quantified using rRT-PCR targeting the E gene in swab samples.
Biomarker: SARS-CoV-2
Specimen:
nasopharyngeal swab and oropharyngeal swab
Units: cycle threshold
Gene target: E
Participants: 2
Negative samples: 12
Positive samples (not quantifiable): 0
Quantifiable samples: 14
Limit of quantification: 35
Limit of detection: unknown
sputum_SARSCoV2_E_Ct
Cycle threshold (Ct) values were quantified using rRT-PCR targeting the E gene in sputum samples.
Biomarker: SARS-CoV-2
Specimen:
sputum
Units: cycle threshold
Gene target: E
Participants: 2
Negative samples: 12
Positive samples (not quantifiable): 0
Quantifiable samples: 8
Limit of quantification: 35
Limit of detection: unknown
sputum_SARSCoV2_RdRp_Ct
Cycle threshold (Ct) values were quantified using rRT-PCR targeting the RdRp gene in sputum samples.
Biomarker: SARS-CoV-2
Specimen:
sputum
Units: cycle threshold
Gene target: RdRp
Participants: 2
Negative samples: 10
Positive samples (not quantifiable): 0
Quantifiable samples: 10
Limit of quantification: 35
Limit of detection: unknown
swab_SARSCoV2_RdRp_Ct
Cycle threshold (Ct) values were quantified using rRT-PCR targeting the RdRp gene in swab samples.
Biomarker: SARS-CoV-2
Specimen:
nasopharyngeal swab and oropharyngeal swab
Units: cycle threshold
Gene target: RdRp
Participants: 2
Negative samples: 11
Positive samples (not quantifiable): 0
Quantifiable samples: 15
Limit of quantification: 35
Limit of detection: unknown