Real-time quantitative PCR detection of four human bocaviruses kantola2010real
Study developing and analytically validating multiplex and singleplex real-time quantitative PCR (qPCR) assays for detecting and quantifying human bocaviruses (HBoV1-4). The assays were applied to stool swab extracts from 250 Finnish patients whose stool samples were submitted for diagnosis of gastrointestinal infection; 5 samples were reproducibly positive (4 HBoV2, 1 HBoV3). Table 4/CSV provide individual-level stool bocavirus DNA loads (copies/mL of fecal extract) for the 5 positive patients along with age, sex, and travel history/other findings.
Analytes
stool
Human bocavirus (HBoV) DNA quantification in stool extract using multiplex screening real-time qPCR followed by species-discriminating singleplex qPCR assays (HBoV2, HBoV3; HBoV1-4 assay platform described). Stool swabs were suspended in Tris-EDTA buffer, boiled, nucleic acids purified (NucliSENS easyMAG), and eluate tested by TaqMan-based qPCR with FAM-labeled MGB hydrolysis probe targeting the left-hand UTR/beginning of NS1 region. Clinical results reported as copies/mL of fecal extract.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.