Infectious human adenoviruses are shed in urine even after disappearance of urethral symptoms hanaoka2019infectious

Comparative study in Japan (urology STI clinic, June 2014–May 2016) of male patients with acute urethritis and asymptomatic male volunteers. First-void urine (FVU) samples were screened for urethritis-related pathogens including human adenovirus (HAdV). HAdV DNA was detected by real-time and nested PCR and quantified by real-time PCR as copies/mL urine; infectious virus was assessed by culture isolation in A549 cells with confirmation by cytopathic effect and PCR. This extraction captures individual longitudinal urine HAdV DNA measurements (copies/mL) from a CSV derived from figures/tables, timed by days from symptom onset.

Analytes

urine_adenovirus_viral_load

Quantification of human adenovirus (HAdV) DNA in first-void urine using real-time PCR (with HAdV detection also performed by real-time and nested PCR in the study). Values reported as copy number per mL urine (copies/mL). Virus isolation in A549 cells was also performed in the study but is not part of this numeric analyte.

Biomarker: adenovirus
Specimen: urine
Units: gc/mL
Participants: 32
Negative samples: 15
Positive samples (not quantifiable): 0
Quantifiable samples: 57
Limit of quantification: unknown
Limit of detection: unknown