Onset and window of SARS-CoV-2 infectiousness and temporal correlation with symptom onset: a prospective, longitudinal, community cohort study hakki2022onset

Through a prospective, longitudinal, community cohort study that captures the critical growth phase and peak of viral replication, the goal is to characterize the window of SARS-CoV-2 infectiousness and its temporal relationship with symptom onset.

Analytes

asymptomatic_PCR

This analyte represents the detection and quantification of SARS-CoV-2 viral RNA from throat and nose swabs specimens collected from asymptomatic participants. The analysis focuses on measuring the viral load expressed in log10 copies/mL, with the primary reference event being the enrollment.

Biomarker: SARS-CoV-2
Specimen: nasopharyngeal swab and oropharyngeal swab
Units: gc/mL
Participants: 17
Negative samples: 96
Positive samples (not quantifiable): 0
Quantifiable samples: 146
Limit of quantification: unknown
Limit of detection: unknown

asymptomatic_cultivable

The infectious virus analyte consisted of culturable SARS-CoV-2 derived from throat and nasal swabs of asymptomatic participants, measured using a plaque assay in vitro. The unit of measurement was PFU/mL, with enrollment as the primary reference event.

Biomarker: SARS-CoV-2
Specimen: nasopharyngeal swab and oropharyngeal swab
Units: pfu/mL
Participants: 17
Negative samples: 190
Positive samples (not quantifiable): 0
Quantifiable samples: 52
Limit of quantification: unknown
Limit of detection: unknown

symptomatic_PCR

This analyte represents the detection and quantification of SARS-CoV-2 viral RNA from throat and nose swabs specimens collected from symptomatic participants. The analysis focuses on measuring the viral load expressed in log10 copies/mL, with the primary reference event being the symptom onset.

Biomarker: SARS-CoV-2
Specimen: nasopharyngeal swab and oropharyngeal swab
Units: gc/mL
Participants: 33
Negative samples: 125
Positive samples (not quantifiable): 0
Quantifiable samples: 347
Limit of quantification: unknown
Limit of detection: unknown

symptomatic_cultivable

The infectious virus analyte consisted of culturable SARS-CoV-2 derived from throat and nasal swabs of symptomatic participants, measured using a plaque assay in vitro. The unit of measurement was PFU/mL, with symptom onset as the primary reference event.

Biomarker: SARS-CoV-2
Specimen: nasopharyngeal swab and oropharyngeal swab
Units: pfu/mL
Participants: 33
Negative samples: 294
Positive samples (not quantifiable): 0
Quantifiable samples: 178
Limit of quantification: unknown
Limit of detection: unknown