Nosocomial acute gastroenteritis outbreak caused by an equine-like G3P[8] DS-1-like rotavirus and GII.4 Sydney[P16] norovirus at a pediatric hospital in Rio de Janeiro, Brazil, 2019 gutierrez2021nosocomial

This study investigates a nosocomial outbreak of acute gastroenteritis caused by rotavirus and norovirus at a pediatric hospital in Rio de Janeiro, Brazil, in May 2019. The outbreak affected 30 individuals, including children and hospital staff. Nine stool samples were analyzed using RT-qPCR to detect and quantify the viruses. The study identified the circulation of equine-like G3P[8] DS-1-like rotavirus and GII.4 Sydney[P16] norovirus. The study emphasizes the importance of active surveillance and prevention measures to control nosocomial infections in hospital settings.

Analytes

stool_rotavirus_viral_load

Detection and quantification of rotavirus in stool samples using RT-qPCR targeting the NSP3 gene segment.

Biomarker: rotavirus
Specimen: stool
Units: gc/wet gram
Gene target: NSP3
Participants: 4
Negative samples: 0
Positive samples (not quantifiable): 0
Quantifiable samples: 4
Limit of quantification: unknown
Limit of detection: unknown

stool_norovirus_viral_load

Detection and quantification of norovirus in stool samples using RT-qPCR targeting the ORF1/2 junction region.

Biomarker: norovirus
Specimen: stool
Units: gc/wet gram
Gene target: ORF1/2
Participants: 5
Negative samples: 0
Positive samples (not quantifiable): 0
Quantifiable samples: 5
Limit of quantification: unknown
Limit of detection: unknown

stool_rotavirus_ct

Ct value for rotavirus detection in stool samples using RT-qPCR targeting the NSP3 gene segment.

Biomarker: rotavirus
Specimen: stool
Units: cycle threshold
Gene target: NSP3
Participants: 4
Negative samples: 0
Positive samples (not quantifiable): 0
Quantifiable samples: 4
Limit of quantification: unknown
Limit of detection: unknown

stool_norovirus_ct

Ct value for norovirus detection in stool samples using RT-qPCR targeting the ORF1/2 junction region.

Biomarker: norovirus
Specimen: stool
Units: cycle threshold
Gene target: ORF1/2
Participants: 5
Negative samples: 0
Positive samples (not quantifiable): 0
Quantifiable samples: 5
Limit of quantification: unknown
Limit of detection: unknown