Prospective study of human metapneumovirus infection: diagnosis, typing and virus quantification in nasopharyngeal secretions from pediatric patients gerna2007prospective

Prospective evaluation (winter-spring 2004-2005 and 2005-2006) of human metapneumovirus (hMPV) detection/typing in pediatric patients using monoclonal antibodies and RT-PCR, with viral load quantification in nasopharyngeal secretions. CSV-extracted figure data provide per-patient day-0 and follow-up measurements (relative to symptom onset) for metapneumovirus and some co-detected respiratory viruses (rhinovirus, influenza, coronavirus).

Analytes

metapneumovirus

Quantification of human metapneumovirus (hMPV) RNA in nasopharyngeal secretions by RT-PCR (exact assay details not available in provided text; values provided as viral load).

observations for metapneumovirus

Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.

Biomarker: metapneumovirus
Specimen: nasopharyngeal aspirate
Units: gc/mL
Participants: 11
Negative samples: 11
Positive samples (not quantifiable): 0
Quantifiable samples: 11
Limit of quantification: unknown
Limit of detection: 1000

rhinovirus

Detection/quantification of rhinovirus RNA in nasopharyngeal secretions by RT-PCR (exact assay details not available in provided text; values provided as viral load).

observations for rhinovirus

Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.

Biomarker: rhinovirus
Specimen: nasopharyngeal aspirate
Units: gc/mL
Participants: 2
Negative samples: 0
Positive samples (not quantifiable): 0
Quantifiable samples: 2
Limit of quantification: unknown
Limit of detection: unknown

influenza

Detection/quantification of influenza virus in nasopharyngeal secretions by RT-PCR (exact assay details not available in provided text; value reported as negative in CSV).

Biomarker: influenza
Specimen: nasopharyngeal aspirate
Units: gc/mL
Participants: 1
Negative samples: 0
Positive samples (not quantifiable): 1
Quantifiable samples: 0
Limit of quantification: 1000
Limit of detection: unknown

hCoV_63

Detection/quantification of hCoV NL63 in nasopharyngeal secretions by RT-PCR (exact assay details not available in provided text; value reported as negative in CSV).

Biomarker: hCoV NL63
Specimen: nasopharyngeal aspirate
Units: gc/mL
Participants: 1
Negative samples: 0
Positive samples (not quantifiable): 1
Quantifiable samples: 0
Limit of quantification: 1000
Limit of detection: unknown