Infectious viral shedding of SARS-CoV-2 Delta following vaccination: A longitudinal cohort study garciaknight2022infectious
Longitudinal cohort study (San Francisco Bay Area, Sep 2020–Oct 2021) comparing nasal SARS-CoV-2 RNA and infectious virus shedding in 84 non-hospitalized adults (52 unvaccinated, 32 fully vaccinated). Participants self-collected anterior nasal swabs daily for up to 14 days and intermittently to day 28. RT-qPCR quantified N and E gene copies/mL using plasmid standards; viral culture (CPE) and plaque assays on Vero-hACE2-TMPRSS2 cells assessed infectious virus. Time values are reported relative to symptom onset. The provided CSV contains per-participant quantitative RT-qPCR measurements for N and E (copies/mL) and vaccination category.
Analytes
e
RT-qPCR quantification of SARS-CoV-2 envelope (E) gene from anterior nares swabs. Absolute quantification used a 10-fold serial dilution of plasmid standards containing E and N genes; reactions run on a CFX Connect system. Samples were self-collected anterior nasal swabs stored in VTM and processed as described in methods. Values reported as copies per mL derived from the standard curve.
Fitted by censored maximum likelihood. The red line is the median individual; the shaded region is the full range of a simulated cohort drawn from the fitted population, so it shows what simulating from this dataset would produce rather than a confidence interval, with dashed lines at the central 95%. Open triangles are non-detects, drawn at the censoring limit and entering the fit as "below this value" rather than being dropped. See the modelling methods for what these estimates do and do not support.
n
RT-qPCR quantification of SARS-CoV-2 nucleocapsid (N) gene from anterior nares swabs. Absolute quantification used a 10-fold serial dilution of plasmid standards containing N and E genes; reactions run on a CFX Connect system. Samples were self-collected anterior nasal swabs stored in VTM and processed as described in methods. Values reported as copies per mL derived from the standard curve.
Fitted by censored maximum likelihood. The red line is the median individual; the shaded region is the full range of a simulated cohort drawn from the fitted population, so it shows what simulating from this dataset would produce rather than a confidence interval, with dashed lines at the central 95%. Open triangles are non-detects, drawn at the censoring limit and entering the fit as "below this value" rather than being dropped. See the modelling methods for what these estimates do and do not support.