Development of real-time PCR assays for detection and quantification of human bocavirus choi2008development
Study developing and clinically evaluating TaqMan real-time PCR assays targeting human bocavirus (HBoV) NS1, NP-1, and VP1 genes. Clinical evaluation tested 506 nasopharyngeal (nasal) aspirates collected in Korea from Dec 2006 to May 2007 from patients with acute respiratory tract infections; 11 patients were positive by all three assays and had quantified viral loads reported as copies per mL of specimen.
Analytes
nasopharyngeal_aspirate_bocavirus_viral_load
Quantification of human bocavirus (HBoV) in nasal aspirate specimens by TaqMan MGB probe real-time PCR (ABI Prism 7900HT). Three assays targeting NS1, NP-1, and VP1 were run; viral load in clinical positives reported as mean copies/mL across the three primer–probe sets from three replicate tests. DNA extracted from 200 µL specimen using MagNa Pure LC total nucleic acid extraction kit. Real-time PCR reaction: 20 µL with 2 µL extracted DNA, TaqMan Universal PCR Master Mix, 900 nM primers, 250 nM probe; cycling 50°C 2 min (UNG), 95°C 10 min, 40 cycles 95°C 15 s and 60°C 1 min.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.