Molecular epidemiology of human metapneumovirus in Ireland carr2008molecular

Pediatric respiratory specimens collected in Ireland were screened for human metapneumovirus (hMPV). An indirect immunofluorescence assay was validated against a quantitative real-time RT-PCR assay, and viral loads (copies/mL) were quantified for 30 hMPV-positive respiratory specimens; genotypes included A2a, A2b, and B2 subclusters (B2a, B2b).

Analytes

respiratory_metapneumovirus_viral_load

Quantitative real-time reverse-transcriptase PCR (real-time RT-PCR) assay used to detect and quantify human metapneumovirus (hMPV) in pediatric respiratory specimens; values reported as copies/mL (quantitative determinations). Indirect immunofluorescence (IF) was also used for screening/validation, but the extracted measurements are RT-PCR viral loads.

observations for respiratory_metapneumovirus_viral_load

Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.

Biomarker: metapneumovirus
Specimen: nasopharyngeal aspirate
Units: gc/mL
Gene target: N
Participants: 30
Negative samples: 0
Positive samples (not quantifiable): 0
Quantifiable samples: 30
Limit of quantification: unknown
Limit of detection: unknown