Influenza and respiratory syncytial virus screening for the detection of asymptomatically infected patients in hematology and oncology baier2018influenza
Retrospective analysis of a prophylactic seasonal RT-PCR screening program (Dec 2016 to Apr 2017) for influenza A/B and RSV among hospitalized asymptomatic patients on pediatric and adult hemato-oncological wards at Hannover Medical School (Germany). Respiratory specimens (mostly nasopharyngeal swabs; some pharyngeal lavage/oral wash) were tested by real-time RT-PCR and viral load changes were assessed semi-quantitatively using PCR cycle threshold (Ct) values. The CSV-provided data include longitudinal Ct values over days after the initial positive screening timepoint for 6 asymptomatic patients (5 influenza A; 1 RSV).
Analytes
respiratory_influenza_ct
Semi-quantitative influenza viral load measured as real-time RT-PCR cycle threshold (Ct). RNA extracted (QIAamp Viral RNA Mini Kit on QIAcube). Detection via influenza A/B multiplex real-time RT-PCR (differentiates types A and B). Ct values used for follow-up comparison; Ct decrease by ≥4 interpreted as significant viral load increase.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.
respiratory_RSV_ct
Semi-quantitative RSV viral load measured as real-time RT-PCR cycle threshold (Ct). RNA extracted (QIAamp Viral RNA Mini Kit on QIAcube). Detection via commercially available one-step real-time RT-PCR kit (RSV/hMPV r-gene PCR Kit, bioMérieux). Ct values used for follow-up comparison; Ct decrease by ≥4 interpreted as significant viral load increase.
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.