Longitudinal and quantitative fecal shedding dynamics of SARS-CoV-2, pepper mild mottle virus, and crAssphage arts2023longitudinal

Concentration of RNA of the N gene quantified using (RT)-ddPCR in stool samples. The concentration was quantified in gene copies per dry weight of stool. The limit of blank (LOB), determined as the upper 95% confidence limit of the negative extraction control, ranged from 11.2 to 1,550 gc/mg-dry weight. The reported number is either the measured concentration of SARS-CoV-2 N or the LOB if the concentration wasn’t detectable.

Concentration of RNA of the ORF1a gene quantified using (RT)-ddPCR in stool samples. The concentration was quantified in gene copies per dry weight of stool. The limit of blank (LOB), determined as the upper 95% confidence limit of the negative extraction control, ranged from 11.2 to 1,550 gc/mg-dry weight. The reported number is either the measured concentration of SARS-CoV-2 ORF1a or the LOB if the concentration wasn’t detectable.

Concentration of PMMoV RNA was measured by ddPCR on the same day as the SARS-CoV-2. The concentration was quantified in gene copies per dry weight of stool. The reported number is either the measured concentration of PMMoV or the LOB if the concentration wasn’t detectable.

Concentration of crAssphage DNA was measured using qPCR. The reported number is either the measured concentration of crAssphage or the Limit of Quantification (LOQ) if the concentration wasn’t quantifiable.