Detection of Norovirus in Saliva Samples from Acute Gastroenteritis Cases and Asymptomatic Subjects: Association with Age and Higher Shedding in Stool anfrunsestrada2020detection
Outbreak investigation study in Catalonia (Spain) during 2017–2018 assessing human norovirus (HuNoV) RNA detection/quantification in saliva (and paired stool for many participants) from symptomatic acute gastroenteritis cases and asymptomatic exposed subjects in closed/semi-closed institutions. Saliva HuNoV screening/quantification was performed by RT-qPCR following ISO 15216-1:2017, with genogroup-specific limits of quantification reported for GI and GII; the figure-derived CSV provided here contains individual saliva GII measurements over days relative to symptom onset (as used for symptomatic individuals in the paper).
Analytes
saliva_gii
Human norovirus genogroup II (GII) RNA in saliva quantified by RT-qPCR according to ISO 15216-1:2017 after RNA extraction from 0.2–0.5 mL saliva; saliva collected by spitting 2–3 mL into sterile tube and stored at −20 °C until testing. The paper reports a genogroup-specific limit of quantification (LOQ) for saliva of 2.95 log10 genome copies/mL for GII (i.e., ~8.91e2 gc/mL).
Measurements only — no shedding model is fitted to this analyte, usually because it is sampled once per participant, leaving no trajectory to fit, or because nothing was ever detected. Open triangles are non-detects, drawn at the assay's censoring limit. See the modelling methods for what these estimates do and do not support.