N1_probe
SARS-CoV-2 RNA genome copy concentration calculated from evaluation of both N1 probe. Results were considered positive if signals were detected (Ct < 40) for the RP, N1, and N2 genes. Results were classified as ‘not detected’ if RP was detected but no signal was observed (Ct >= 40) from either N1 or N2. Results were classified as inconclusive if RP was detected (Ct < 40) and either N1 or N2 was detected (but not both). Results were classified as invalid if no RP was detected in the sample. Any specimens for which results were inconclusive were retested; specimens which produced inconclusive results after retesting were excluded from the analysis (n = 17 specimens from 6 participants). Ct values for amplification of both viral targets (N1 and N2 probes). But for further analysis, the author focused on N1 probe